EZ Cap PEX-EGFP Probe mRNA (m1Ψ)

EZ Cap PEX-EGFP Probe mRNA (m1Ψ)

Author: ApexBio

shortsummary:

Peroxisomes (PEX) probes mRNA with EGFP, modified by N1-Methylpseudo-UTP (m1Ψ), providing higher transcription efficiency and suppressing RNA-mediated innate immune activation.

63-89-8

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Dry ice

description:

PEX-EGFP is a fusion protein, consisting of Peroxisome (PEX) targeting signal sequence and enhanced green fluorescent protein (EGFP). EGFP is a modified green fluorescent protein with higher brightness and stability, suitable for live cell imaging. By fusing EGFP with the PEX signal sequence, the dynamic behavior, distribution, and function of PEX can be observed in real time in living cells, helping to reveal the key role of PEX in signal transduction, cell metabolism, and related diseases. EZ Cap PEX-EGFP Probe mRNA (m1Ψ) is provided at a concentration of ~1 mg/ml with Cap1 structure. It is specifically used for the expression of EGFP markers in peroxisomes. There are currently two ways to cap mRNA: One is co-transcription method, by adding Cap analogues into the transcription process. The other is enzymatic Capping. After transcription, Cap0 capping is performed by Vaccinia virus Capping Enzyme (VCE), GTP and S-adenosylmethionine (SAM). The Cap0 is then generated into the Cap1 through 2´-O-Methyltransferase and SAM. Cap1 Capping can also be performed by adding VCE, 2´-O-Methyltransferase, GTP and SAM in a one-step process. Cap 1 structure is more ideal for mammalian systems and possess higher transcription efficiency than Cap 0 structure. The addition of N1-Methylpseudo-UTP(m1Ψ) and poly(A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly(A) tail also plays an important role in enhancing the efficiency of translation initiation.

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APExBIO Technology LLC is a premier provider of Small Molecule Inhibitors/Activators, Compound Libraries, Peptides, Assay Kits, Fluorescent Labels, Enzymes, Modified Nucleotides, mRNA synthesis and various tools for Molecular Biology. We carry a broad product line in over 25865 different research areas such as cancer, immunology, neurosciences, apoptosis and epigenetics etc. Based in USA (Houston, Texas), we have been serving the needs of customers across the world.